Agilent 1260 Infinity G1364C Analytical-Scale Fraction Collector
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The Agilent 1260 Infinity G1364C Analytical-Scale Fraction Collector is designed for optimized fraction collection at flow rates between 100 μL/min and 10 mL/min. This module fits within any Infinity LC stack and is ideal for compound purification in the nanogram to milligram range using columns with internal diameters between 2.1 and 9.4 mm. Unique fraction delay sensor technology determines fraction delay volumes automatically and ensures that fractions are collected just-in-time without the need to collect extra volume.
S/N: DEACT00707
Specifications:
| Type | Specifications |
|---|---|
| Trigger modes | Time slices, Peak (threshold, up- / downslope), Timetable (combination of time intervals and peak) and Manual trigger (supported only with Agilent Instant Pilot G4208A)
Agilent 1260 Infinity DAD/MWD detectors (G1315C,D; G4212B/ G1365 C,D), the Agilent 1260 Infinity Fluorescence Detector (G1321A) and the Agilent G1946C/D, G1956A/B LC-MSD are fully supported other detectors can be used but are not supported for fraction collection. |
| Operating modes | Discrete fractions: default mode for all vessels. The flow is diverted to waste, while moving from one vessel position to the next vessel position
Continuous flow: optional, available only when using the deep well plates. It is possible to move from one well plate position to the next one without diverting the flow into the well plate to waste Needle into location: Needle pushes into the vessel as deep as specified, for the use with capped vials and test tubes and well plates with closing mats Droplet setup mode: The tip of the fraction collector needle will initially move down to the bottom of the well. Then it will slowly move upwards while the fraction is collected. The droplet setup mode enables the fraction collector to collect small fractions without bubbles. |
| Fraction vessel capacities and trays | 4 x well-plates full tray (MTP)*
2 x well-plates std. tray + 10 funnels with external containers* (+ 1 half tray) 2 × well-plates std. tray (MTP) + 10 × 2 ml vials* (+ 1 half tray)•100 x 2 ml in std. tray (+ 1 half tray)* 3 x 40 x 2 ml in half tray* 3 x 40 funnels in half tray 3 x 15 x 6 ml in half tray* Full tray with 40 test tubes (30 mm OD, max. height 48 mm, ~20 ml vol.) Full tray with 60 test tubes (25 mm OD, max. height 48 mm) Full tray with 126 test tubes (16 mm OD, max. height 48 mm) Full tray with 215 test tubes (12 mm OD, max. height 48 mm) Installed trays are automatically detected and identified. Installed plates and vials can be detected when operating in the needle into location mode * max. height can be extended by using the short needle assembly G1364-87202 |
| Maximum tube / plate height | 48 mm with long needle assembly G1367-87200
75 mm with short needle assembly G1364-87202 |
| Maximum tube volume | ca. 20 ml with 48 mm test tubes, ca. 30 ml with 75 mm test tubes or unlimited, if funnels are used with external containers. |
| Maximum flow rate | 10 ml / min (depending on viscosity and generated back pressure, max. 6 bar at the diverter valve). The analytical scale fraction collector can be modified for flow rates > 10 ml/min. |
| Delay volumes [μl] | Fraction collector inlet to diverter valve: ~50 (typical, depends on the length of the tubing)
Diverter valve: ~15 Diverter valve to needle: ~10 Needle: ~4 |
| Delay calibration sensor | Single wavelength absorbance detector working at 654 nm, consisting of a LED and a photo diode |
| Diverter valve | 3/2 Diverter valve with low internal volume (15 μl), switching time < 100 ms, maximum operating pressure 6 bar |
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