Agilent 1200 Series G1377A Micro Well-Plate Autosampler

Type

Specification

GLP features

Early maintenance feedback (EMF), electronic records of maintenance and errors

Communications

Communications Controller-area network (CAN). RS232C, APG-remote standard, optional four external contact closures and BCD vial number output

Safety features

Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display

Injection range

0.01– 8 µl in 0.01 µl increments with the small loop capillary 0.01–40 µl in 0.01 µl increments with the extended loop capillary

Precision

Typically < 0.5% RSD of peak areas from 5 – 40 µl, Typically < 1% RSD from 1 – 5 µl Typically < 3% RSD from 0.2 – 1 µl

Pressure range

400 bar (5880 psi)

Sample viscosity range

0.2 – 5 cp

Sample capacity

2 × well-plates (MTP) + 10 × 2 ml vials

108 x 2-mL vials in 2 x 54 vial plate plus 10 additional 2 mL vials

30 x 6-mL vials in 2 x 15 vial plate plus 10 additional 2 mL vials

54 Eppendorf tubes (0.5/1.5/2.0 mL) in 2 x 27 Eppendorf tube plate

Injection cycle time

Typically < 30 s using following standard conditions: Default draw

speed: 4 µl/min Default eject speed: 10 µl/min Injection volume:

0.1 µl

Carry-over

Typically < 0.05% using the following conditions: Column:

150 x 0.5 mm Hypersil ODS, 3 µm Mobile phase:

Water/Acetonitrile = 85/15 Column Flow rate: 13 µl/min Injection

volume: 1 µl caffeine (=25 ng caffeine), 1 µl water to test carryover

Outside wash of needle before injection: 20 sec with water using flush port