Dionex Summit HPLC System With P680, ASI-100, PDA-100 and TCC-100
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This Dionex 680 HPLC System includes:
- P680 HPLC Pump
- ASI-100 Automated Sample injector
- PDA-100 Photodiode Array Detector
- TCC-100 Thermostatted Column Compartment
- PC with software
P680 HPLC Pump
| Specification | |
| Flow rate range | 1-10,000 µL/min |
| Flow rate accuracy and reproducibility | ±0.1% at 1 mL/min |
| Pressure range | 0.1–50 MPa (7250 psi) |
| Pressure ripple | Typically <1% |
| Proportioning accuracy | ±0.5% at 2 mL/min |
| Proportioning reproducibility | ±0.5% at 2 mL/min |
Dionex ASI-100 Automated Sample injector
| Specification | |
| Injection principle | In-line split-loop |
| Sample capacity | Depending on the vial carrier; carrier with:
3 anal./cylind. segments = 117 vials at 1.8 ml + 3 at 4 ml 3 anal./concial segments = 117 vials at 1.1 ml + 3 at 4 ml 3 mini segments = 192 vials at 1.2 ml + 3 at 4 ml 3 mini segments/Beckmann = 192 vials at 0.25ml + 3 at 4 ml 3 semiprep. segments = 63 vials at 4 ml + 3 at 4 ml 3 Eppendorf segments = 66 vials at 2.0 ml + 3 at 4 ml 3 Eppendorf/concial segments = 66 vials at 1.5 ml + 3 at 4 ml 3 Eppendorf/conical segments = 66 vials at 0.5 ml + 3 at 4 ml |
| Vial types | Depending on the segment type:
Type 01 (Analyt./Cylindrical): 1.8 ml vials, cylindrical, with crimp cap Type 01 (Analyt./Conical): 1.1 ml vials, conical, with crimp cap Type 02 (Mini): 1.2 ml vials, cylindrical, with crimp cap Type 03 (Semiprep): 4 ml vials, cylindrical, with screw cap All above vials with silicone/PTFE septa. Type 02 (Mini/Beckmann): 0,25 ml Beckmann mini vials (Beckmann part no. 652823) Type 04 (Eppendorf): 2.0 ml Eppendorf vials (Eppendorf part no. 0030 120.094) Type 04 (Eppendorf/Conical): 1.5 ml Eppendorf vials, conical (Eppendorf part no. 0030 120.086) Type 04 (Eppendorf/Conical): 0.5 ml Eppendorf vials, conical (Eppendorf part no. 0030 121.023) |
| Injection Volume | ASI-100/ASI-100T: 1 – 250 µl in 0.1µl increments
ASI-100P/ASI-100PT: 10 – 2500 µl in 1 µl increments |
| Carry-Over | < 0.1% |
| Linearity | RSD < 0.5 % |
| Flush volume | None |
| Sample loss/injection | None |
| Min. required sample | 5µl (ASI-100/ASI-100T) |
| Reproducibility | Specified RSD < 0.3% at 5µl (250 µl syringe)
Typical RSD < 0.2% at 5 µl 250 µl syringe) Typical RSD < 1.0% at 1µl 250 µl syringe) Specified RSD < 0,3% at 100 µL (2500 µl syringe) |
| Repeat injections | Max. 9 per vial |
| Counters | For a description of the various counters that provide information about the autosampler status |
| Method Parameters | For a detailed description of the method parameters |
| Safety features | Overflow/leak sensor, automatic segment detection (when enabled), tray detection/manual interference monitoring (when enabled), automatic vial recognition (i.e., monitoring in case no vial is present at the specified position), electronic needle protection, all mechanical operations internally monitored (injection valve, syringe etc.) |
| Temperature Control
(ASI-100T/ ASI-100PT) |
In the range of 4 – 45°C (39.2 – 113°F)
cooling: -18°C (32.4°F) from ambient heating: +35°C (63°F) from ambient |
| Input/Display | LCD, keypad with control elements and function keys for direct control, LED display (7 segments) for retention time indication, LED display (7 segments) plus three LEDs (red/green/blue)
indicating the sample position, 2 LEDs (Load/Inject) indicating the status of the motorized switching valve, 2 LEDs (cool/heat) indicating the temperature status (ASI-100T/ASI-100PT) |
| Wetted parts | Stainless steel (1.4571/316SS), PCTFE, PTFE, PEEK, Vespel |
| Operating pressure | Max. 340 bar (34 MPa) Max 200 bar (20 MPa) with 2.5 ml syringe + corresponding sample loop |
| In-/Outputs | 2x serial (RS232[A] and RS232 [B]), MSV, Sample Position, Digital I/O |
Dionex PDA-100 Photodiode Array Detector
| Specification | |
| Optical System | Single-beam, reverse-optics design with concave holographic Grating |
| Light Sources | Deuterium lamp (30 W) for ultraviolet spectrum analysis Tungsten lamp (15 W) for visible spectrum analysis |
| Photodiode Array | 1024 elements |
| Wavelength Range | 190 nm to 800 nm |
| Pixel Resolution | 0.7 nm |
| Wavelength Accuracy | ± 1 nm |
| Noise | ± 10 µAU with 2-second rise time, 254 nm, 4 nm bandwidth, and flowing deionized water
± 15 µAU with 2-second rise time, 520 nm, 10 nm bandwidth, and flowing deionized water |
| Drift | < 500 µAU/hour (after warm-up) |
| Linearity | > 2 AU |
| Analog Outputs | Four built-in analog outputs |
| Voltage Out | 1 V output |
| Analog Output Range | 0.0005, 0.001, 0.01, 0.1, 0.5, 1.0, 2.0, or 3.0 AU full-scale |
| Control and DataEvaluation | Provided by PeakNet 6 or Chromeleon software; communication with the detector is via the DX-LAN interface |
| Cell Body | PEEK or stainless steel |
| Volume and Optical Path Length | PEEK cell: 13 µL volume; 10 mm (0.394 in) path length
Stainless steel cell: 13 µL volume; 10 mm (0.394 in) path length |
| Maximum Operating Pressure | PEEK cell: 2 MPa (300 psi)
Stainless steel cell: 3 MPa (500 psi) |
| Chemical Compatibility | Do not use bases stronger than 0.1 M; they will etch the silica windows of the flow cells. Do not use normal phase or chlorinated solvents with the PEEK flow cell; they will damage the cell Do not use high pH salts with the stainless steel flow cell; they will cause corrosion, contamination, and metal leaching |
Dionex TCC-100 Thermostatted Column Compartment
| Specification | |
| Environmental
Conditions |
Range of use: Indoor use
Temperature: 10°C to 35°C (50°F to 95°F) Air humidity: 40 to 85% rel. humidity, non-condensing Overvoltage Category: II Polution degree: 2 |
| Operating Conditions | Temperature range: +5°C to +85°C (+41°F to +185°F)
When cooling: max. 15°K from ambient |
| Temperature Setting | In 0.1°C increments |
| Temperature Accuracy | ± 0.5°C |
| Temperature Stability | ± 0.1°C |
| Temperature Precision | ± 0.1°C |
| Heat-up/Cool-down Time | Less than 20 minutes from 20°C to 50°C
Less than 20 minutes from 50°C to 20°C average change per minute: 2-4°K |
| Column Capacity | 3 columns (maximum length: 30 cm) |
| Control | All functions controllable via LAN or USB
Permanent status transfer Program-controlled by Chromeleon or stand-alone operation |
| Switching Valve | Options: 2-position/6-port or 2-position/10-port switching valve
Maximum backpressure: 345 bar (5000 psi) Switching time: 110 ms |
| GLP | When controlled by Chromeleon:
Dionex AutoQ routines for Instrument Qualification Column identification system for 2 columns |
This refurbished Dionex P680 HPLC System is in great condition. It has been tested at SpectraLab Scientific.
A 90-day warranty is included.
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