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Dionex UltiMate 3000 VWD-3400 Variable Wavelength Detector

Item ID:

DNX-VWD-3400

Manufacturer

Condition

Refurbished

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The VWD-3400 Variable Wavelength Detector is a high-quality instrument designed for HPLC analysis as part of a Thermo Fisher/ Dionex UltiMate 3000 system. The module was developed especially for HPLC analysis and can be used in numerous laboratory environments for routine analysis and sophisticated HPLC research tasks.

Specifications:

Type Specifications
Optical design Dual-beam photometer (monochromator)

Multiple wavelength UV/VIS detector

Light source Deuterium lamp and tungsten lamp

Temperature control for both lamps

Wavelength range 190-900 nm

The tungsten lamp is recommended for wavelengths > 600 nm.

Noise Single wavelength:

<± 3.5 μAU (typical <± 2,5 μAU):

dry analytical flow cell, wavelength: 254 nm, time constant (according to ASTM): 1 second, only deuterium lamp turned on

<± 5 μAU (typical):

wet analytical flow cell, wavelength: 254 nm, time constant (according to ASTM): 1 second, only deuterium lamp turned on, flowing HPLC grade methanol at 1.0 mL/min

<± 5 μAU (typical):

dry analytical flow cell, wavelength: 520 nm, time constant (according to ASTM): 1 second, only tungsten lamp turned on <± 7.5 μAU (typical): wet analytical flow cell, wavelength: 520 nm, time constant (according to ASTM): 1 second, only tungsten lamp turned on, flowing HPLC grade methanol at 1.0 mL/min

All values reached within 60 minutes of warm-up (typical).

Multiple wavelength:

<± 25 μAU (typical: <± 10 μAU):

dry analytical flow cell, wavelengths: 254 nm and 280 nm, time constant: 2 seconds, data rate: 2 Hz, only deuterium lamp turned on

<± 30 μAU (typical: <± 10 μAU):

dry analytical flow cell, wavelengths: 450 nm and 520 nm, time constant: 2 seconds, data rate: 2 Hz, only tungsten lamp turned on

<± 50 μAU (typical: <± 10 μAU):

wet analytical flow cell, wavelengths: 450 nm and 520 nm, time constant: 2 seconds, data rate: 2 Hz, only tungsten lamp turned on, flowing HPLC grade methanol at 1.0 mL/min

All values reached within 60 minutes of warm-up (typical).

Drift < 1 x 10-4 AU/hour:

dry analytical flow cell, wavelength: 254 nm, only deuterium lamp turned on, constant ambient conditions (temperature, humidity)

Typical < 1 x 10-4 AU/hour:

dry analytical flow cell, wavelength: 520 nm, only tungsten lamp turned on, constant ambient conditions (temperature, humidity)

Typicial < 2 x 10-4 AU/hour after 60 minutes warm-up

Linearity <5% at 2.5 AU caffeine, wavelength: 272 nm, based on ASTM (correlation coefficient > 99.97%, <3% RSD up to 2.5AU)

<5% at 2.5 AU cobalt, wavelength: 520 nm (correlation coefficient > 99.95%, <3% RSD up to 2.5AU)

Wavelength calibration Internal calibration with zero order and D-alpha line of the deuterium lamp
Wavelength verification Internal verification with holmium oxide filter
Data collection rate Single wavelength:

Adjustable, up to 100 Hz (VWD-3400) or 10 Hz (VWD-3100)

Multiple wavelengths:

Adjustable, up to max. 5 Hz, depending on the number of wavelengths (2-4) and the distance between the wavelengths (in nm)

Wavelength accuracy ± 1 nm (over detector lifetime)
Wavelength repeatability ± 0.1 nm
Optical bandwidth 6 nm at 254 nm
Flow cells Analytical standard flow cell:

Path length: 10 mm; cell volume: 11 μL;

pressure limit: 120 bar (stainless steel), 50 bar (PEEK)

Optional flow cells for nano, cap, micro, and semipreparative HPLC

Identification of flow cell type and serial number via built-in ID chip

Control All parameters and functions software controlled, USB

3 LEDs (Power, Connected, Status) for status monitoring

I/O interfaces 4 digital input, 4 digital outputs
Analog output 2 analog outputs available as an option via DAC plug-in module software selectable: absorbance, 20 bit resolution, 0-1V (full range) and 0-10 V (full range with adjustable mAU ranges).
Spectrum scanning Yes, with different scan speeds
GLP features In Chromeleon:

Full support of automatic equipment qualification (AutoQ) and system performance monitoring.

All system parameters are logged in the Audit Trail.

Safety feature Power-up diagnostics of optics, cooling fans, motors, and electronics Leak sensor
Wetted parts PEEK, quartz glass, stainless steel
Environmental conditions Range of use: Indoor use Temperature: 10 °C to 35°C Air humidity: 80% relative humidity, non-condensing Overvoltage category: II Pollution degree: 2
Dimensions (h × w × d) 16 × 42 × 51 cm

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