Thermo Scientific Fluoroskan Ascent FL Microplate Fluorometer and Luminometer
3-12 month warranty
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PN: 5210450
The Thermo Scientific Fluoroskan Ascent FL is a microplate fluorometer and luminometer which offers versatility and flexibility for even the most demanding fluorometric applications. The extended model is equipped with both fluorometric and luminometric detection technologies. As a combination instrument it covers the full range of fluorometric as well as glow and flash luminometric applications.
The advanced optical system based on direct illumination optics, produces a sharply focused light beam for fluorometric measurements, prevents crosstalk and ensures accurate readings. The choice of two beam diameter settings allows optimal reading of 1 to 96 and 384 wells. High sensitivity is one of the main benefits of fiberless optics and a critical feature for luminometry.
Specifications
| Weight | Basic unit 21 kg (46 lbs.).
3 optional dispensers add 3.5 kg to the weight. |
| Overall dimensions | 420 mm (16.5 in.) (W) x 420 mm (16.5 in.) (D) x 340 mm (13.4 in.) (H), options included. |
| Operating conditions (indoor use) | +10°C – +40°C, RH 90% max.
Tested according to IEC 60068-2-1 test Ab, (Cold). IEC 60068-2-2 test Bb, (Dry heat). IEC 60068-2-3 test Ca, (Damp heat). |
| Transportation conditions | -40°C – +70°C, packed in transportation packaging.
Tested according to IEC 60068-2-1 test Ab, (Cold). IEC 60068-2-2 test Bb, (Dry heat). |
| Storage conditions | -25°C – +50°C, packed in transportation packaging.
Tested according to IEC 60068-2-1 test Ab, (Cold). IEC 60068-2-2 test Bb, (Dry heat). |
| Mains power supply | 100 – 240 Vac, 50/60 Hz, nominal |
| Power consumption | 200 VA max., 32 VA standby. |
| Heat dissipation | 683 BTU max. |
| Computer interface | Serial RS-232C port. Baud rate 9600. Character format 1 start bit, 8 data bits, 1 stop bit, no parity. Flow control XON/XOFF. |
| Light source | Quartz-halogen lamp. Power consumption 30 W. |
| Detector | Photomultiplier tube. |
| Fluorescence reference | Lisa KL 3-9402 Polymethylmethacrylate. |
| Filters for fluorometric measurements | Both the excitation and emission filter wheels can comprise max. eight selectable filters (Fluoroskan Ascent). However, in the Fluoroskan Ascent FL, eight excitation and six emission filters can be installed into the filter wheels, since the emission filter slots 7 and 8 are always reserved for luminometric measurements.
Transmittance 30 – 100%, half-bandwidth 6 – 50 nm, depending on the wavelength. |
| Filters included in the instrument | Excitation filters: 355 nm, and 485 nm.
Emission filters: 460 nm, and 538 nm. |
| Filters for luminometric measurements | Filters can be used. Luminometric filters and fluorometric emission filters are situated in the same filter wheel, maximum 6 filters. |
| Plate types | 1- to 384-well plates. Can also be programmed for nonstandard configurations. Maximum dimensions 90 mm x 134 mm x 25 mm. |
| Shaker | Built-in orbital shaker with adjustable speed and diameter. |
| Incubator | Temperature range from RT (25°C) +3°C to 45°C, when the ambient temperature is 25°C. The temperature is selected using Ascent Software. |
| Dispensers | 1 – 3 optional dispensers. Syringe volumes 1000 µl.
Dead volume 600 µl. Metal-free fluid path. Exchangeable valve, syringe, tubing and tip. Autoclavable tubing and tip. |
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