Thermo Scientific Orbitrap Elite with LTQ Velos Pro and ETD

This system includes:

• (1) Thermo Scientific Velos Pro
• (1) Thermo Scientific Orbitrap Elite
• (1) Thermo Scentific Neslab ThermoFlex 900
• (1) Thermo Scientific ETD
• (2) Rough pump
• (1) PC with software
• (Optional) HPLC front end

Thermo Scientific Velos Pro

• Resolving power up to 100,000 FWHM and mass accuracy better than 1ppm RMS provide confidence when analyzing complex samples
• Multiple fragmentation techniques—CID, HCD, and optionally ETD—offer versatility and facilitate structural characterization
• Fast scanning and parallel MS and MSn analysis speeds analyses
• Ion optics with novel neutral-blocking technology increase robustness
• Collision cell with axial field gradient improves quantitative results
• High mass resolution and mass accuracy enable high-confidence molecular weight determination of intact proteins, peptide identification in complex samples and analysis of isobaric species
• High sensitivity increases detection of low-abundance peptides and proteins
• Short duty cycle increases sequence coverage within in a single experiment
• Stability, mass accuracy and wide dynamic facilitate label-free quantification
• Mass resolution and mass accuracy distinguish between heavy and light peptide ion pairs and interferences in SILAC experiments
• HCD cell with axial field improves quantification of isobarically labeled peptides
• Optional electron-transfer dissociation (ETD) generates complementary information for P™ analyses and de novo sequencing

Thermo Scientific Orbitrap Elite

Analyze demanding proteomics, metabolomics, lipidomics and metabolism samples in greater depth using the Thermo Scientific™ Orbitrap Elite™ Hybrid Ion Trap-Orbitrap Mass Spectrometer. This instrument combines a high-field Thermo Scientific™ Orbitrap™ mass analyzer and Thermo Scientific™ Velos Pro™ ion trap technology to deliver very high resolution, speed, sensitivity and complementary fragmentation information.

Thermo Scentific Neslab ThermoFlex 900 

Optimized for the most demanding applications, Thermo Scientific™ ThermoFlex Recirculating Chillers are reliable, easy-to-use and easy-to-maintain. The ThermoFlex platform was developed with customer input from concept to design. This high-performance chiller platform, with cooling capacity up to 24,000 watts (81,948 btu/hr), is configurable to meet application demands.

Thermo Scientific ETD

Achieve better analysis of proteins and their post-translational modifications (PTMs) with the Thermo Scientific™ Electron Transfer Dissociation (ETD) option. Unlike CID, which cleaves weakly bound modifications off the peptide backbone, ETD induces fragmentation along the peptide backbone in a sequence-independent manner, leaving labile modifications linked to the peptide chain. This greatly simplifies identification of modification sites. ETD produces primarily c- and z-type fragment ions that complement the b- and y-type ions produced by CID, increasing sequence coverage and protein IDs.

• ETD preserves labile PTMs, allowing straightforward identification of the peptide sequence and the site of modification
• For top-down analysis, ETD can be combined with proton transfer reaction (PTR) which dramatically simplifies spectra from intact proteins
• For workflows that include low-mass isobaric mass tags, ETD can be combined with pulsed-Q dissociation (PQD) which eliminates the normal low-mass cutoff
• ETD can be added to most Thermo Scientific LTQ-series ion trap and hybrid ion trap-Orbitrap mass spectrometers
• Proteome Discoverer software includes the proprietary Z-Core database search algorithm specifically optimized for ETD spectra
• Proteome Discoverer software can easily merge complementary CID and ETD results to maximize protein sequence coverage