Triple Quadrupole Operation, Troubleshooting & Maintenance

This is an excellent hands-on training course in LC-MS/MS, a rapidly growing technique, designed to introduce instrument operation, troubleshooting and maintenance of an LC/MS System.  Modern equipment is used in conjunction with practical aspects providing strong grounding in LC-MS/MS instrumental techniques. A brief outline of the program is as detailed below:

• Overview of LC-MS, Single and Triple-Quad instrumental techniques
• HPLC Considerations for LC-MS/MS
• Flow Rate and Flow Splitting Considerations
• Electrospray Ionisation (ESI) Theory & Instrumentation
• Atmospheric Pressure Ionisation (API) Theory & Instrumentation
• Calibrating and Tuning
• Tuning and Optimising the ESI Interface Parameters
• Choosing API Mode, Alternative Sources and Multi-mode Sources
• Designing Spectral Experiments – Scan, SIM and MRM Acquisition for Qualitative and Quantitative Operation
• Quantitative Analysis using the MS Detector (Calibration, LOD, etc.)
• Qualitative Analysis using the MS Detector (In-house Libraries/Peak ID and Library Searching)
• In-Source Collision Induced Dissociation (CID)
• Introduction to Spectral Interpretation
• MS Diagnostics and Introduction to LC-MS Routine Maintenance

Course Type: Hands-On
Level: Intermediate
Duration: 2 days
Equipment:
Option 1: PE Sciex API 4000/3000/2000/365 or Sciex 2000 Qtrap LC/MS/MS System*
Training Code: TC-MS-0004/001

Option 2: Thermo Finnigan LCQ Classic/Duo/Deca LC/MS/MS System*
Training Code: TC-MS-0004/002

Option 3: Thermo Finnigan TSQ 7000 or TSQ Quantum LC/MS/MS System
Training Code: TC-MS-0004/003

Option 4: Micromass Quattro LC/MS/MS System
Training Code: TC-MS-0004/004

Option 5: Varian 1200L Quadrupole LC/MS/MS System
Training Code: TC-MS-0004/005