Whey proteins, including β-lactoglobulin, α-lactalbumin, and bovine serum albumin, are essential components of dairy products and nutritional supplements due to their high biological value and functional properties. Precise separation and quantification of these proteins are vital for quality control, nutritional analysis, and product formulation in the food and biopharmaceutical industries.Benzene is a volatile aromatic compound often present as a trace contaminant in hydrocarbon solvents such as toluene, xylene, and hexane. Even at very low levels, benzene poses serious health and safety concerns due to its toxicity and carcinogenic nature. Monitoring benzene concentration in solvents is therefore essential for ensuring product purity, regulatory compliance, and worker safety in chemical manufacturing, laboratory, and industrial applications.