Agilent 1200 Series Rapid Resolution HPLC System

The design concept of the 1200 Rapid Resolution LC (RRLC) System was to provide a liquid chromatograph offering ultra fast and high resolution separation capability and yet which retained full functionality for standard HPLC applications. This section looks at how this concept has been implemented to offer the highest flexibility with respect to column dimensions and applications.

The use of sub-two micron (STM) particles means that for high flowrates or long columns additional pressure is required to drive the mobile phase through the column. The RRLC flowpath is optimized to produce minimal backpressure and ZORBAX RRHT columns have an engineered particle size distribution that produces significantly less backpressure than other STM columns. High temperature, up to 100°C on certain columns, allows more selectivity flexibility and reduces solvent viscosity to allow even faster separation. High flow rates up to 5 ml/min can be used for ultra-fast separations. The adjustable delay volume fully supports 2.1 to 4.6 mm i.d. columns. A low dispersion tubing kit and low volume flow cells minimize peak dispersion for narrow bore columns. Detectors with high data rates preserve the resolution of very fast peaks eluting from the RRLC.

System Includes:

• G1379B Micro Vacuum Degasser
• G1312B Binary Pump SL
• G1367C High Performance HiP Autosampler SL
• (optional) G1330B Autosampler Thermostat
• G1316B Thermostatted Column Compartment SL
• G1315C Diode Array Detector SL (DAD) or G1314E Variable Wavelength Detector SL+ (VWD)