New Thermo Orbitrap Fusion Lumos Tribrid MS in original crate

This Orbitrap is brand new and in its original crate. We can offer a complete system with any UHPLC front end.

Excel at the most challenging of applications, including low level PTM analysis, multiplexed relative quantitation using isobaric tags, intact protein characterization, as well as MSn analysis of small molecules with the new Thermo Scientific Orbitrap Fusion Lumos Tribrid Mass Spectrometer. This system incorporates the brightest ion source, a segmented quadrupole mass filter with improved selectivity and ion transmission, Advanced Vacuum Technology for improved ion transmission to the Thermo Scientific Orbitrap mass analyzer, and ETD HD, a higher-capacity ETD fragmentation. Latest innovations include the Advanced Peak Determination (APD) algorithm for improved data-dependent experiments and two new hardware options: UVPD, a new fragmentation technique achieved with a 213 nm UV laser and 1M, which provides 1,000,000 FWHM ultra-high resolution for improved structural elucidation and quantitation of isobaric compounds.

Achieve better analysis of proteins and their post-translational modifications (PTMs) with the Thermo Scientific Electron Transfer Dissociation (ETD) option. Unlike CID, which cleaves weakly bound modifications off the peptide backbone, ETD induces fragmentation along the peptide backbone in a sequence-independent manner, leaving labile modifications linked to the peptide chain. This greatly simplifies identification of modification sites. ETD produces primarily c- and z-type fragment ions that complement the b- and y-type ions produced by CID, increasing sequence coverage and protein IDs.

• Electron transfer dissociation is a supremely useful tool for the analysis of post-translationally modified proteins. ETD capability can be added to many Thermo Scientific ion trap and Orbitrap mass spectrometers. Thermo Scientific Proteome Discoverer software includes unique capabilities to help users take full advantage of the information provided by ETD.
• ETD preserves labile PTMs, allowing straightforward identification of the peptide sequence and the site of modification
• For top-down analysis, ETD can be combined with proton transfer reaction (PTR) which dramatically simplifies spectra from intact proteins
• For workflows that include low-mass isobaric mass tags, ETD can be combined with pulsed-Q dissociation (PQD) which eliminates the normal low-mass cutoff
• ETD can be added to most Thermo Scientific LTQ-series ion trap and hybrid ion trap-Orbitrap mass spectrometers
• Proteome Discoverer software includes the proprietary Z-Core database search algorithm specifically optimized for ETD spectra
• Proteome Discoverer software can easily merge complementary CID and ETD results to maximize protein sequence coverage

Orbitrap Fusion Lumos Tribrid MS features:

• Novel high-sensitivity API interface combines a High Capacity Transfer Tube and an Electrodynamic Ion Funnel for increased ion flux and lower limits of detection
• Advanced Active Beam Guide to prevent neutrals and high velocity clusters from entering the resolving quadrupole
• Advanced Quadrupole Technology combines high selectivity and efficiency of transfer for selected ions symmetrically across the isolation window
• Advanced Vacuum Technology improves transmission of high molecular weight ions to the Orbitrap analyzer
• Novel ETD HD—high dynamic range ETD provides significantly increased fragment ion coverage
• Advanced Peak Determination (APD) – a new algorithm for improved precursor determination in data-dependent experiments, resulting in a large increase in identification of unique peptides and experimental throughput
• Tribrid architecture includes a quadrupole mass filter, linear ion trap and an Orbitrap mass analyzer for maximal duty cycle due to a high degree of parallelization of various instrument operations
• Ultrahigh resolving power up to 500,000 FWHM, with isotopic fidelity up to 240,000 FWHM at m/z 200
• Acquisition rates of up to 20 Hz for both Orbitrap and linear ion trap MSn analyses
• Full parallelization of MS and MSn analyses with intelligent ADAPT (All Dynamically Available Parallelizable Time) technology
• Synchronous Precursor Selection (SPS) for MS and MSn experiments significantly increases the number of peptides and proteins identified and improves quantitative accuracy when using isobaric mass tags
• Flexibility of fragmentation — CID, HCD and optional ETD and EThcD available at any stage of MSn with detection in either the Orbitrap or linear ion trap detector enables detailed structural determination of metabolites, glycans, and other small molecules
• Universal Method provides maximal peptide identification without method optimization for samples of unknown concentration, reducing sample and instrument time requirements for routine peptide identification experiments
• Intuitive and flexible drag-and-drop user interface simplifies method development and enables unique and complex workflows